Publications

2026

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  Shimakawa G, Nam O, Schober AF, Mueller-Cajar O, Mackinder LCM, Matsuda Y (2026). From seawater to pyrenoid: the molecular architecture of the diatom CO₂-concentrating mechanism. Plant Physiology 201(4):kiag607.

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  #Mihris Ibnu Saleem Naduthodi, #James Barrett, Jessica Pritchard, Philippe Van der Stappen, Manon Demulder, Tommaso Garfagnini, Adam Dowle, Maria Hondele, Benjamin D. Engel, Alistair McCormick, Luke C. M. Mackinder (2026). Molecular organization of the Chlorella sorokiniana pyrenoid. bioRxiv. Preprint

  • Authors marked with # contributed equally
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  James Barrett, Clément Dégut, Alejandro Sebiani Calvo, Michael R. Hodgkinson, Hannah Derry, Aidan Salter, Emma Coulembier Vandelannoote, Rebecca Beveridge, John H. Bothwell, Thomas B. Jacobs, Olivier De Clerck, James N. Blaza, Michael J. Plevin, Luke C.M. Mackinder, Jonas Blomme (2026). A spatial atlas of the seaweed CO₂-fixation machinery reveals a unique Rubisco condensation mechanism. bioRxiv. Preprint

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  Dao O, Barrett J, Burlacot A, Peltier G, Li‐Beisson Y, Mackinder LCM (2026). The mosaic of aquatic photorespiration: evolution, diversity, and global biogeochemical impacts. New Phytologist 251(6):3122-3141.

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  Adler L, Lau CS, Esch L, Dao O, Barrett J, Grant S, Yates G, McCormick AJ, Mackinder LCM (2026). EPOS1 is required for normal pyrenoid starch sheath formation and efficiency of the CO₂‐concentrating mechanism in Chlamydomonas reinhardtii. New Phytologist 251(5):2518-2531.

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  Nam O, Grouneva I, Mackinder LCM (2026). A versatile tool for gene editing in the diatom Thalassiosira pseudonana. Plant Physiology.

• Wunder T, Eulitz L, Kramer L, Ali ZM, Ostermeier M, Leu C, Szulc B, Holzner LJ, Fechter J, Padovani F, Brandt B, Girr P, Teh JT, Mühlbauer S, Sotos C, Angstenberger M, Mackinder LCM, Schmoller KM, Rädler JO, Nickelsen J, de Vries J, Kunz HH (2026). Conserved and lineage-specific roles of KEA-mediated ion homeostasis in Chlamydomonas. Plant Physiology 201(3):kiag405.

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  Barrett J#, Nam O#, Naduthodi MIS#, Mackinder LCM (2026). Pyrenoid Structure, Function, Evolution, and Characterization Across Diverse Lineages. Annual Review of Plant Biology 77(1):81-115.

  • Authors marked with # contributed equally

• Pritchard JH, Young SNR, Garland H, Mackinder LCM, McCormick AJ (2026). Pyrenoid-based CO₂-concentrating mechanisms across diverse lineages: convergent features and their applications for engineering. Plant Physiology.

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  Kumar G#, Barrett J#, Van der Stappen P#, Payne-Dwyer A, Nam O, Shepherd J, Frame L, Howard J, Malis V, Degut C, Hodgkinson M, Richter R, Schaefer C, Plevin M, Hondele M, Leake M, Engel B, Mackinder L (2026). Sticker number modulates pyrenoid condensate assembly to support algal fitness. bioRxiv 2026.01.27.701992. Preprint

  • Authors marked with # contributed equally

2025

• Inckemann RM, Chotel T, Burgis M, Brinkmann CK, Andreas L, Baumann J, Sharma P, Klose M, Barrett J, Ries F, Paczia N, Glatter T, Mackinder LCM, Willmund F, Erb TJ (2025). A modular high-throughput approach for advancing synthetic biology in the chloroplast of Chlamydomonas. Nature Plants 11(11):2332-2349.

• Dao O, Bertrand M, Alseekh S, Veillet F, Auroy P, Nguyen PC, Légeret B, Epting V, Morin A, Cuiné S, Monteil CL, Mackinder LCM, Burlacot A, Krieger-Liszkay A, Weber APM, Fernie AR, Peltier G, Li-Beisson Y (2025). The green algae CO₂ concentrating mechanism and photorespiration jointly operate during acclimation to low CO₂. Nature Communications 16(1):5296.

• Shaikh KM, Walker CE, Tóth D, Kuntam S, Polgár TF, Petrova NZ, Garland H, Mackinder LCM, Tóth SZ, Spetea C (2025). The thylakoid-and pyrenoid-localized phosphate transporter PHT4-9 is essential for photosynthesis in Chlamydomonas. Plant Physiology 198(1):kiaf158.

• Emrich-Mills TZ, Proctor MS, Degen GE, Jackson PJ, Richardson KH, Hawkings FR, Buchert F, Hitchcock A, Hunter CN, Mackinder LCM, Hippler M, Johnson MP (2025). Tethering ferredoxin-NADP+ reductase to photosystem I promotes photosynthetic cyclic electron transfer. The Plant Cell 37(3):koaf042.

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  Perrin AJ, Dowson M, Davis K, Nam O, Dowle AA, Calder G, Springthorpe VJ, Zhao G, Mackinder LCM (2025). CyanoTag: Discovery of protein function facilitated by high-throughput endogenous tagging in a photosynthetic prokaryote. Science Advances 11(6):eadp6599.

2024

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  Catherall E, Musiał S, Atkinson N, Walker CE, Mackinder LCM, McCormick AJ (2024). From algae to plants: understanding pyrenoid-based CO₂-concentrating mechanisms. Trends in Biochemical Sciences 50(1):33-45.

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  Barrett J, Naduthodi MIS, Mao Y, Degut C, Musiał S, Salter A, Leake MC, Plevin MJ, McCormick AJ, Blaza JN, Mackinder LCM (2024). A promiscuous mechanism to phase separate eukaryotic carbon fixation in the green lineage. Nature Plants 10(11):1801-1813.

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  Nam O, Musiał S, Demulder M, McKenzie C, Dowle A, Dowson M, Barrett J, Blaza JN, Engel BD, Mackinder LCM (2024). A Protein Blueprint of the Diatom CO₂-Fixing Organelle. Cell 187(21):5935-5950.e18.

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  Adler L, Lau CS, Shaikh KM, van Maldegem K, Payne-Dwyer AL, Lefoulon C, Girr P, Atkinson N, Barrett J, Emrich-Mills T, Dukic E, Blatt MR, Leake M, Peltier G, Spetea C, Burlacot A, McCormick AJ, Mackinder LCM, Walker CE (2024). Bestrophin-like protein 4 involved in photosynthetic acclimation to light fluctuations in Chlamydomonas. Plant Physiology 196(4):2374-2394.

• Kim G, Hugonnet H, Kim K, Lee J-H, Lee SS, Ha J, Lee C, Park H, Yoon K-J, Shin Y, Csucs G, Hitchcock I, Mackinder L, Kim JH, Hwang TH, Lee S, O'Toole P, Koo B-K, Guck J, Park Y (2024). Holotomography. Nature Reviews Methods Primers 4(1):51.

• Payne-Dwyer A#, Kumar G#, Barrett J#, Gherman LK, Hodgkinson M, Plevin M, Mackinder L, Leake MC, Schaefer C (2024). Predicting Rubisco-Linker Condensation from Titration in the Dilute Phase. Physical Review Letters 132:218401.

  • Authors marked with # contributed equally

2023

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  Lau CS, Dowle A, Thomas GH, Girr P, Mackinder LCM (2023). A phase-separated CO₂-fixing pyrenoid proteome determined by TurboID in Chlamydomonas reinhardtii. The Plant Cell 35(9):3260-3279.

• Wostrikoff K, Mackinder LCM (2023). Rubisco and inorganic carbon assimilation. In The Chlamydomonas Sourcebook (Academic Press) Pages 223-271.

• Silflow CD, Mackinder LCM, Wingfield J (2023). Methods for the Localization of Cellular Components in Chlamydomonas. In The Chlamydomonas Sourcebook (Academic Press) Pages 345-384.

2022

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  Nam O#, Grouneva I#, Mackinder LCM (2022). Endogenous GFP tagging in the diatom Thalassiosira pseudonana. bioRxiv 2022.09.30.510313. Preprint

  • Authors marked with # contributed equally

2021

• Barrett J#, Girr P#, Mackinder LCM (2021). Pyrenoids: CO₂-fixing phase separated liquid organelles. Biochimica et Biophysica Acta (BBA) - Molecular Cell Research 1868(5):118949.

  • Authors marked with # contributed equally

• Emrich-Mills T, Yates G, Barrett J, Grouneva I, Lau CS, Walker CE, Kwok TK, Davey JW, Johnson MP, Mackinder LCM (2021). A recombineering pipeline to clone large and complex genes in Chlamydomonas. The Plant Cell 33:1161-1181.

2020

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  Meyer MT, Itakura AK, Patena W, Wang L, He S, Emrich-Mills T, Lau CS, Yates G, Mackinder LCM, Jonikas MC (2020). Assembly of the Algal CO₂-Fixing Organelle, the Pyrenoid, Is Guided by a Rubisco-Binding Motif. Science Advances 6(46):eabd2408.

• Ramundo S, Asakura Y, Salomé PA, Strenkert D, Boone M, Mackinder LCM, Takafuji K, Dinc E, Rahire M, Crèvecoeur M, Magneschi L, Schaad O, Hippler M, Jonikas M, Merchant S, Nakai M, Rochaix JD, Walter P (2020). Coexpressed subunits of dual genetic origin define a conserved supercomplex mediating essential protein import into chloroplasts. PNAS 117:32739–32749.

2019

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  Mukherjee A, Lau CS, Walker CE, Rai A, Prejean CI, Yates G, Emrich-Mills T, Lemoine SG, Vinyard DJ, Mackinder LCM, Moroney JV (2019). Thylakoid localized bestrophin-like proteins are essential for the CO₂ concentrating mechanism of Chlamydomonas reinhardtii. PNAS 116:16915–20.

2018

• Küken A, Sommer F, Yaneva-Roder L, Mackinder LCM, Höhne M, Geimer S, Jonikas MC, Schroda M, Stitt M, Nikoloski Z, Mettler-Altmann T (2018). Effects of microcompartmentation on flux distribution and metabolic pools in Chlamydomonas reinhardtii chloroplasts. eLife 7:e37960.

2017

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  Mackinder LCM (2017). The Chlamydomonas CO₂-concentrating mechanism and its potential for engineering photosynthesis in plants. New Phytologist 217(1):54-61.

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  Mackinder LCM, Chen C, Leib R, Patena W, Blum SR, Rodman M, Ramundo S, Adams C, Jonikas MC (2017). A spatial interactome reveals the protein organization of the algal CO₂ concentrating mechanism. Cell 171:133-147.

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  Freeman Rosenzweig ES, Xu B, Kuhn Cuellar L, Martinez-Sanchez A, Schaffer M, Strauss M, Cartwright HN, Ronceray P, Plitzko JM, Förster F, Wingreen NS, Engel BD, Mackinder LCM, Jonikas MC (2017). The Eukaryotic CO₂-Concentrating Organelle is Liquid-Like and Exhibits Dynamic Reorganization. Cell 171:148-162.

2016

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  Mackinder LCM, Meyer MT, Mettler-Altmann T, Chen V, Mitchell MC, Caspari O, Freeman Rosenzweig ES, Pallesen L, Reeves G, Itakura A, Roth R, Sommer F, Geimer S, Mühlhaus T, Schroda M, Goodenough U, Stitt M, Griffiths H, Jonikas MC (2016). A repeat protein links Rubisco to form the eukaryotic carbon concentrating organelle. Proceedings of the National Academy of Sciences 113:5958-5963.

2015

• Atkinson N, Feike D, Mackinder L, Meyer MT, Griffiths H, Jonikas MC, Smith AM, McCormick AJ (2015). Introducing an algal carbon-concentrating mechanism into higher plants: location and incorporation of key components. Plant Biotechnology Journal 14(5):1302-1315.

• Yang W, Wittkopp TM, Li X, Warakanont J, Dubini A, Catalanotti C, Kim RG, Nowack ECM, Mackinder LCM, Aksoy M, Dudley Page M, D'Adamo S, Saroussi S, Heinnickel M, Johnson X, Richaud P, Alric J, Boehm M, Jonikas MC, Benning C, Merchant S, Posewitz M, Grossman AR (2015). Critical role of Chlamydomonas reinhardtii ferredoxin-5 in maintaining membrane structure and dark metabolism. Proceedings of the National Academy of Sciences 112:14978-14983.

• Müller MN, Barcelos e Ramos J, Schulz KG, Riebesell U, Kaźmierczak J, Gallo F, Mackinder L, Li Y, Nesterenko PN, Trull TW, Hallegraeff GM (2015). Phytoplankton calcification as an effective mechanism to alleviate cellular calcium poisoning. Biogeosciences 12:6493-6501.

2014

• Yang W, Catalanotti C, D'Adamo S, Wittkopp TM, Ingram-Smith CJ, Mackinder L, Miller TE, Heuberger AL, Peers G, Smith KS, Jonikas MC, Grossman AR, Posewitz MC (2014). Alternative acetate production pathways in Chlamydomonas reinhardtii during dark anoxia and the dominant role of chloroplasts in fermentative acetate production. The Plant Cell 26:4499-4518.

• Avasthi P, Onishi M, Karpiak J, Yamamoto R, Mackinder L, Jonikas M, Sale WS, Shoichet B, Pringle JR, Marshall WF (2014). Actin is required for IFT regulation in Chlamydomonas reinhardtii. Current Biology 24:2025-2032.

2013

• Read B, Kegel J, Klute MJ, et al. (2013). Pan genome of the phytoplankton Emiliania underpins its global distribution. Nature 499:209-213.

  • As member of Emiliania huxleyi Annotation Consortium

• Bach L*, Mackinder L*, Schulz K, Wheeler G, Schroeder D, Brownlee C, Riebesell U (2013). Dissecting the impact of CO₂ and pH on the mechanisms of photosynthesis and calcification in the coccolithophore Emiliania huxleyi. New Phytologist 199:121-134.

  • Authors marked with * contributed equally

2011

• Mackinder L, Wheeler G, Schroeder D, von Dassow P, Riebesell U, Brownlee C (2011). Expression of biomineralization-related ion transport genes in the coccolithophore Emiliania huxleyi. Environmental Microbiology 13:3250-3265.

2010

• Mackinder L, Wheeler G, Schroeder D, Riebesell U, Brownlee C (2010). Molecular Mechanisms Underlying Calcification in Coccolithophores. Journal of Geomicrobiology 27:585-595.

2009

• Mackinder L, Worthy CA, Biggi G, Hall M, Ryan KP, Varsani A, Harper G, Wilson WH, Brownlee C, Schroeder D (2009). A unicellular algal virus Emiliania huxleyi virus 86 exploits an animal-like infection strategy. Journal of General Virology 90:2306-16.

• Highfield A, Nagar A, Mackinder L, Noel L, Hall M, Martin S, Schroeder D (2009). Deformed Wing Virus Implicated in Overwintering Honeybee Colony Losses. Applied and Environmental Microbiology 75:7212-7220.